ABSTRACT
Objective To observe the effects of alprostadil injection on intestinal mucosal barrier function in patients with acute pancreatitis(AP). Methods Seventy-eight AP patients admitted in Department of Medicine in Yuhang District Second People's Hospital of Hangzhou City from May 2016 to March 2017 were divided into 2 groups using random number method,including 38 cases in the control group and 40 cases in the treatment group. Patients in the control group received routine treatment. Patients in the treatment group were given intravenous 10 μg/d alprostadil injection in addition to routine treatment continuously for 14 days. Another 40 healthy volunteers who underwent routine examination in the same period were enrolled as healthy control group. The scores of MCTSI and APACHEⅡwere recorded at admission and 2 days after the treatment. The levels of serum DAO, endotoxin (ET) and IFABP were detected by ELISA. Results There were no significant difference on the scores of MCTSI and APACHEⅡ, the levels of serum DAO, ET, IFABP before treatment between control and treatment group(P>0.05), but were increased significantly than those in the healthy control group(P<0.01). After treatment,the scores of MCTSI and APACHEⅡ,the levels of serum DAO, ET, IFABP were decreased significantly than those before treatment in two groups(P<0.05 or P<0.01), but the scores of MCTSI and APACHEⅡ, the levels of serum DAO, ET and IFABP were decreased significantly than those in the control group(3.78 ± 0.43)vs (5.89 ± 0.13),(5.65 ± 1.77)vs (9.05 ± 1.61),(2.18 ± 0.16)U/ml vs (3.22 ± 0.15)U/ml,(0.15 ± 0.06)EU/ml vs (0.25 ± 0.09)EU/ml,(62.01 ± 12.82)ng/L vs (85.43 ± 16.79)ng/L; all P<0.05). There was a significant positive correlation between serum DAO, ET, IFABP and the scores of MCTSI (P<0.01). There was a significant positive correlation between serum DAO, ET, IFABP and the scores of APACHEⅡ, too (P<0.01). Conclusions Patient with acute pancreatitis have obvious intestinal mucosal barrier injury. Alprostadil injection can improve clinical symptoms and the scores of MCTSI to a certain extent by protecting intestinal mucosal barrier function.
ABSTRACT
Objective To investigate the dynamical changes of serum SOCS-3 in patients with acute pancreatitis (AP) and discuss the potential clinical significance.Methods Seventy-five patients with AP admitted in Yuhang District Second People's Hospital of Hangzhou City from February 2015 to December 2016 were selected,who were divided into 2 groups according to disease severity:40 cases in mild AP (MAP) group and 35 cases in moderate and severe AP (MSAP + SAP) group.The levels of serum SOCS-3,IL-6 and TNF-α were determined by enzyme linked immunosorbent assay (ELISA) on the 1,3,5 and 7 day after admission.Thirty healthy people who were age and gender matched were included in the normal control group.Results The levels of serum SOCS-3,IL-6 and TNF-α in AP patients on the 1 day after admission were significantly increased than that in the control group (P ＜ 0.01 or P ＜ 0.05),which were gradually increased with the time,peaked on the 5 day and then started to decrease on 7 day after admission which was still higher than that on the 1 day after admission.The levels of serum SOCS-3,IL-6 and TNF-α in MSAP + SAP group were significantly higher than that in MAP group (P ＜0.01),and there were significant differences (F =112.80,P =0.001;F =170.21,P =0.000;F =112.82,P =0.000).Significant differences were also found among different time points between two groups (F =258.38,P =0.000;F =4.82,P =0.000;F =5.52,P =0.001).Additionally,the significantly positive correlations of SOCS-3 with IL-6 and TNF-α were found (r=0.785,r=0.828,both P＜0.01).Conclusions SOCS-3 may participate in early excessive inflammatory reactions in AP.Dynamic detection of SOCS-3 may be helpful for AP clinical classification and prognosis evaluation.